Green and economical production of propionic acid by Propionibacterium freudenreichii CCTCC M207015 in plant fibrous-bed bioreactor |
| |
Authors: | Feng Xiaohai Chen Fei Xu Hong Wu Bo Li Hui Li Sha Ouyang Pingkai |
| |
Institution: | a State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing 210009, PR China b College of Food Science and Light Industry, Nanjing University of Technology, Nanjing 210009, PR China c State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, PR China |
| |
Abstract: | Propionic acid production by Propionibacterium freudenreichii from molasses and waste propionibacterium cells was studied in plant fibrous-bed bioreactor (PFB). With non-treated molasses as carbon source, 12.69 ± 0.40 g l-1 of propionic acid was attained at 120 h in free-cell fermentation, whereas the PFB fermentation yielded 41.22 ± 2.06 g l-1 at 120 h and faster cells growth was observed. In order to optimize the fermentation outcomes, fed-batch fermentation was performed with hydrolyzed molasses in PFB, giving 91.89 ± 4.59 g l-1 of propionic acid at 254 h. Further studies were carried out using hydrolyzed waste propionibacterium cells as substitute nitrogen source, resulting in a propionic acid concentration of 79.81 ± 3.99 g l-1 at 302 h. The present study suggests that the low-cost molasses and waste propionibacterium cells can be utilized for the green and economical production of propionic acid by P. freudenreichii. |
| |
Keywords: | Propionic acid Cane molasses Waste propionibacterium cell Plant fibrous-bed bioreactor Propionibacterium freudenreichii CCTCC M207015 |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|