Studies on neuraminidase from influenza virus A(H3N2) obtained by two procedures |
| |
| Affiliation: | 1. Department of Viral Ecology, Institut Pasteur, Paris, France;2. Department of Biochemistry. Faculties of Biology and Pharmacy. University of Salamanca and C.S.I.C., Salamanca, Spain;1. Department of Microbiology, The Institute for Viral Diseases, Korea University College of Medicine, Seoul, Republic of Korea;2. Division of Influenza Virus, Korea Centers for Disease Control and Prevention, Osong, Republic of Korea;3. Division of Infectious Diseases, Hallym University College of Medicine, Chuncheon, Republic of Korea;4. Department of Laboratory Medicine, Haeundae Paik Hospital, Inje University College of Medicine, Busan, Republic of Korea;1. Department of Respiratory Medicine, Nagasaki University Hospital, 1-7-1 Sakamoto, Nagasaki City, Japan;2. Infection Control and Education Center, Nagasaki University Hospital, 1-7-1 Sakamoto, Nagasaki City, Japan;3. Department of Neurology and Strokology, Nagasaki University Hospital, 1-7-1 Sakamoto, Nagasaki City, Japan;4. Department of Respiratory Medicine, Sasebo City General Hospital, 9-3 Hirasemachi, Sasebo City, Japan;5. Department of Pathology, Sasebo City General Hospital, 9-3 Hirasemachi, Sasebo City, Japan;6. Department of Laboratory Medicine, Nagasaki University Hospital, 1-7-1 Sakamoto, Nagasaki City, Japan;7. Department of Pathology, Nagasaki University Hospital, 1-7-1 Sakamoto, Nagasaki City, Japan;8. Department of Radiology, Nagasaki University Hospital, 1-7-1 Sakamoto, Nagasaki City, Japan;9. Department of Clinical Nursing, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahata-nishi-ku, Kitakyushu, Fukuoka, Japan;1. Department of Health Promotion Sciences, Maternal and Infant Care, Internal Medicine and Medical Specialties “G. D''Alessandro”, Via del Vespro 133, 90127, University of Palermo, Palermo, Italy;2. Regional Reference Laboratory for Molecular Surveillance of Influenza, Clinical Epidemiology Unit – Via del Vespro 133, 90127, University Hospital “Paolo Giaccone”, Palermo, Italy;1. CHU de Quebec Research Center (CHUL) and Laval University, 2705 Laurier Blvd, Québec City, Quebec, Canada;2. GlaxoSmithKline Vaccines, Laval, Quebec, Canada;1. Department of Microbiology, Nihon University School of Dentistry, Tokyo, 101-8310, Japan;2. Immersion Physics Class, Department of Science, Tokyo Gakugei University International Secondary School, Tokyo, 178-0063, Japan;3. Immersion Biology Class, Department of Science, Tokyo Gakugei University International Secondary School, Tokyo, 178-0063, Japan;1. National Centre of Epidemiology, Institute of Health Carlos III, Spain;2. National Centre for Microbiology, National Influenza Reference Laboratory, WHO-National Influenza Centre, Institute of Health Carlos III, Spain;3. CIBER Epidemiología y Salud Pública (CIBERESP), Institute of Health Carlos III, Spain;4. Subdirección de Salud Pública y Adicciones de Bizkaia, Pais Vasco, Spain;5. Instituto de Salud Pública, Navarra Institute for Health Research (IdiSNA), Pamplona, Spain;6. Dirección General de Salud Pública, Consejería de Sanidad de Castilla y León, Valladolid, Spain;7. Servicio de Epidemiología y Prevención Sanitaria, Dirección General de Salud Pública y Consumo de La Rioja, La Rioja, Spain;8. Servicio de Epidemiología, Dirección General de Salut Pública, Mallorca, Baleares, Spain;9. Servicio de Epidemiología. DGSC, Consejería de Bienestar Social y Sanidad, Ciudad Autónoma de Melilla, Spain |
| |
| Abstract: | - 1.1. Neuraminidase was obtained by (A) bromelain solubilization or (B) by treatment with N-lauroylsarcosine.
- 2.2. 5-N-acetyl-2-O(3-methoxyphenyl)-α-d-neuraminic acid, employed as substrate, avoids the interference produced by the thiobarbituric acid method, and is not interfered by the ampholytes.
- 3.3. Only about 20% of original enzyme activity was lost after electrofocusing. The sample from procedure A showed two peaks, corresponding to pis 4.4 and 5.6. The sample from procedure B, having a higher activity, showed only one peak at pI 4.4.
- 4.4. Samples A and B showed different Km and hydrolysis rate with N-acetylneuraminyl-lactose and glycophorin A. It was not found significantly different with other substrates: α1-acid glycoprotein, brain gangliosides. 5-N-acetyl-2-O-(3-methoxyphenyl)-α-d-neuraminic acid and 2'-(4-methyl umbelliferyl)-α-d-N-acetylneuraminic acid.
|
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
