Determination of urinary free cortisol and cortisone by sequential thin layer and high-performance liquid chromatography |
| |
| Affiliation: | 1. School of Pharmacy, Second Military Medical University, No. 325 Guohe Road, Shanghai 200433, China;2. Department of Health Toxicology, Faculty of Tropical Medicine and Public Health, Second Military Medical University, No. 800 Xiangyin Road, Shanghai 200433, China;3. Department of Pharmacy, the 118th Hospital of PLA, Wenzhou, China;4. Department of Analytical Chemistry and Pharmaceutical Technology, Center for Pharmaceutical Research, Vrije Universiteit Brussel-VUB, Laarbeeklaan 103, B-1090 Brussels, Belgium;5. Laboratory of Analytical Pharmaceutical Chemistry, Department of Pharmaceutical Sciences, University of Liege, CHU B36, B-4000 Liege, Belgium;1. Institute of Biomedical Chemistry, Pogodinskaya Street, 10, Build 8, Moscow, 119121, Russia;2. Pirogov Russian National Research Medical University, Ostrovitianov Street, 1, Moscow, 117997, Russia;3. Institute of Bioorganic Chemistry NASB, 5 Academician V.F. Kuprevich Street, Build 2, Minsk, BY-220141, Belarus;1. Department of Chemistry, School of Chemistry and Materials Science, University of Science and Technology of China (USTC), Hefei, 230026, China;2. State Key Laboratory of Particle Detection and Electronics, University of Science and Technology of China (USTC), Hefei, 230026, China;3. Department of Modern Physics, University of Science and Technology of China (USTC), Hefei, 230026, China;4. National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei, Anhui, 230026, PR China;1. Nanomedicine Lab EA4662, Bat. E, Université de Bourgogne Franche-Comté, UFR Sciences & Techniques, 16 route de Gray, 25030 Besançon Cedex, France;2. Department of Chemistry, College of Science, University of Thi-qar, Thi-qar, Iraq;3. Institut ICMUB - CNRS 6302, Université de Bourgogne Franche-Comté, UFR Sciences et Techniques Mirande, 9 Avenue Alain Savary, 21000 Dijon, France;4. Ecole Polytechnique Fédérale de Lausanne, Section of Chemistry and Chemical Engineering, Station 6, CH-1015 Lausanne, Switzerland;5. Institut FEMTO-ST, UMR CNRS 6174, Department Time-Frequency, 26, Chemin de l''épitaphe, 25030 Besançon Cedex, France;1. Institute of Biomedical Chemistry, Pogodinskaya St. 10/8, 119121, Moscow, Russia;2. Department of Biochemistry, Рeoples’ Friendship University of Russia Named After Patrice Lumumba (RUDN University), Miklukho-Maklaya St. 6, 117198, Moscow, Russia;3. Department of Biochemistry, Pirogov Russian National Research Medical University, Ostrovitianova St. 1, 117997, Moscow, Russia;1. Evotec, Drug Metabolism and Pharmacokinetics Department, Abingdon, Oxon, United Kingdom;2. Evotec, Anti-infective Research Unit, Manchester, United Kingdom;3. Department of Surgery, Massachusetts General Hospital, Boston, MA, United States;4. Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA, United States;5. Shriners Hospitals for Children, Boston, MA, United States;6. Spero Therapeutics, Cambridge, MA, United States |
| |
| Abstract: | A specific, rapid and sensitive method for urinary free cortisol and cortisone utilizing sequential thin layer (TLC) and high-performance liquid chromatography (HPLC) was developed. After addition of prednisone as the internal standard to 1 ml of urine, extraction of the steroids was accomplished by automated sorption on and desorption from a styrenedivinylbenzene copolymeric resin cartridge. Further purification was carried out by TLC. Quantitation of the recovered steroids at 254 nm was achieved during HPLC in a reverse phase system with 21-deoxycortisone as the external standard. When the values of urinary free cortisol both from normal subjects and patients were compared with those obtained by a current RIA procedure, the greater specificity of the new method was clearly demonstrated. Simultaneous measurement of both urinary free cortisol and cortisone in various clinical states appears to offer a more complete index of adrenocortical function than urinary free cortisol alone. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
