Fusion of mitochondria with protoplasts in Saccharomyces cerevisiae. |
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Authors: | Norio Gunge and Kenji Sakaguchi |
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Institution: | (1) Central Research Laboratories, Mitsubishi Chemical Industries, 1000 Kamoshida-cho, Midori-ku, 227 Yokohama, Japan;(2) Mitsubishi-Kasei, Institute of Life Sciences, 11, Minamiooya, Machida-shi, Tokyo, Japan |
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Abstract: | Summary Protoplasts prepared from a neutral petite haploid BO60AF-1 (a ade2 arg4 leu2 trp C
O
E
O
O
O O O) were mixed with mitochondria isolated from an oligomycin resistant respiring haploid ANROR 12D (a his4 leu2 thr4 C
S
E
S
O
II
R
+ +) and treated with 30% polythylene glycol and CaCl2. When the treated protoplasts were spread and incubated on selective agar plates, oligomycin resistant respiration-sufficient colonies appeared with low frequency. All of these colonies carried the mitochondrial genotype of C
S
E
S
O
II
R
+ + and showed the same mating type and nutritional requirements as did BO60AF-1, thus evidencing the mitochondrial transfer into protoplasts. Recombination and transmission of the mitochondrial drug resistance markers were studied in crosses involving the strains issued from mitochondria accepted protoplasts. |
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