A Rapid Silver Impregnation Method for Nervous Tissue: A Modified Protargol-Peroxide Technic |
| |
Authors: | G P Loots J M Loots J M M Brown J L Schoeman |
| |
Institution: |
a Departments of Anatomy and Physiology, University of Pretoria, Pretoria, Republic of South Africa |
| |
Abstract: | A rapid, reliable silver impregnation method is described for nervous tissue fixed in formol-saline, Bouin or Sum. Sections are impregnated for 10-15 minutes at room temperature or 37 C in a solution containing 0.5 g Protargol-S, 0.005-0.01 g allantoin, 1 ml of 1% CuNO2]2, 1 ml of 1% AgNO3. and 1-2 drops of 30% H2O2 in 100 ml distilled water. Thereafter the dons arc reduced in a hydroquinone-formalin solution. This is followed by gold toning and subsequent reduction and mounting. Alternatively. following the first reduction, the silver image can be intensified by placing sections in a silver-allantoin bath which is followed by reduction and mounting. This method is very reliable and selective, making it suitable for general routine and research use. |
| |
Keywords: | |
本文献已被 InformaWorld 等数据库收录! |
|