Proteomic analysis of the reactive phenotype of astrocytes following endothelin-1 exposure |
| |
Authors: | Egnaczyk Gregory F Pomonis James D Schmidt Julie A Rogers Scott D Peters Christopher Ghilardi Joseph R Mantyh Patrick W Maggio John E |
| |
Institution: | Department of Pharmacology and Cell Biophysics, University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA. |
| |
Abstract: | Reactive gliosis is an invariant feature of the pathology of central nervous system (CNS) injury and a major determinant of neuronal survival and regeneration. To begin to understand the alterations in astrocyte protein expression that drive glial changes that occur following injury, we used an established model system (endothelin-1 stimulation of hypertrophy) and proteomic analysis to define a discrete set of differentially expressed proteins and post-translational modifications that occur as the astrocytes change from a quiescent to a reactive state. This orchestrated set of changes included proteins involved in cytoskeletal reorganization (caldesmon, calponin, alpha B-crystallin, stathmin, collapsing response mediator protein-2), cell adhesion (vinculin, galectin-1), signal transduction (RACK-1) and astrocyte differentiation (glutamine synthetase). Using proteomic analysis to understand what drives astrocyte expression of these functionally divergent molecules may offer insight into the mechanisms by which astrocytes can exhibit both pro-regenerative and anti-regenerative activities following CNS injury. |
| |
Keywords: | Astrocyte Cytoskeleton Endothelin Hypertrophy |
本文献已被 PubMed 等数据库收录! |
|