Evaluation of NK-to-target cell binding and evidence for T cell conjugates by flow cytometry |
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Authors: | Marco Vitale Riccardo Rizzoli Adriana R. Mariani Luca M. Neri Andrea Facchini Stefano Papa |
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Affiliation: | (1) Istituto di Anatomia Umana Normale, Bologna, Italy;(2) Istituto di Clinica Medica e Gastroenterologia, Bologna, Italy;(3) Istituto di Scienze Morfologiche, Urbino, Italy;(4) Istituto di Citomorfologia Normale e Patologica, CNR, Italy |
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Abstract: | A flow cytometric methodology was set up to assess the binding capability of peripheral blood NK and T cells to the K562 tumor cell line. Differential side scatter characteristics between effectors and targets were used to analyze conjugated and unconjugated cells. The previous labeling of NK and T cells with anti-Leu 11c and anti-Leu 4 monoclonal antibodies, allowed the distinction between unconjugated non-fluorescent and conjugated fluorescent targets and the percentual evaluation of bound anti-Leu 11c+ and anti-Leu 4+ cells.Abbreviations NK Natural Killer - MoAb Monoclonal Antibody - SSC Side Scatter - FLS Forward Light Scatter - PE Phycoerythrin - MHC Major Histocompatibility Complex - GVHD Graft Versus Host Disease |
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Keywords: | natural killer T lymphocytes flow cytometry side scatter binding conjugates |
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