A Novel Matrix Protein p10 from the Nacre of Pearl Oyster (Pinctada fucata) and Its Effects on Both CaCO3 Crystal Formation and Mineralogenic Cells |
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Authors: | Cen Zhang Shuo Li Zhuojun Ma Liping Xie Rongqing Zhang |
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Institution: | (1) Institute of Marine Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, 100084, People's Republic of China;(2) Protein Science Laboratory of the Ministry of Education, Tsinghua University, Beijing, 100084, People's Republic of China |
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Abstract: | A novel matrix protein, designated as p10 because of its apparent molecular mass of 10 kDa, was isolated from the nacreous
layer of pearl oyster (Pinctada fucata) by reverse-phase high-performance liquid chromatography. In vitro crystallization experiments showed that p10 could accelerate
the nucleation of calcium carbonate crystals and induce aragonite formation, suggesting that it might play a key role in nacre
biomineralization. As nacre is known to contain osteogenic factors, two mineralogenic cell lines, MRC-5 fibroblasts and MC3T3-E1
preosteoblasts, were used to investigate the biological activity of p10. The results showed that p10 could increase alkaline
phosphatase activity, an early marker of osteoblast differentiation, while the viability of MRC-5 and MC3T3-E1 remained unchanged
after treatment of p10. Taken together, the findings led to identification of a novel matrix protein from the nacre of P. fucata that plays a role in both the mineral phase and in the differentiation of the cells involved in biomineralization. |
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Keywords: | Aragonite biomineralization bone formation nacre soluble matrix proteins |
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