Functional mapping in pea, as an aid to the candidate gene selection and for investigating synteny with the model legume Medicago truncatula |
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Authors: | G Aubert J Morin F Jacquin K Loridon M C Quillet A Petit C Rameau I Lejeune-Hénaut T Huguet J Burstin |
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Institution: | (1) Unité de recherche en génétique et écophysiologie des légumineuses, URLEG, INRA, Domaine d’Epoisses, 21110 Bretenières, France;(2) Station de Génétique Végétale, INRA, Domaine Brunehaut, 80200 Peronne, France;(3) Laboratoire de physiologie de la différenciation végétale, USTL, Batiment SN2 3ème étage, 59655 Villeneuve d’Ascq Cedex, France;(4) INRA SGAP, Route de St-Cyr, 78026 Versailles Cedex, France;(5) Laboratoire de Biologie moléculaire des Relations Plantes-Microorganismes, CNRS-INRA, BP 27, 31326 Castanet Tolosan Cedex, France;(6) Present address: Laboratoire Biotechnologie et Amélioration des Plantes, INP-ENSAT, BP 107, 31326 Castanet Tolosan Cedex, France |
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Abstract: | The identification of the molecular polymorphisms giving rise to phenotypic trait variability—both quantitative and qualitative—is
a major goal of the present agronomic research. Various approaches such as positional cloning or transposon tagging, as well
as the candidate gene strategy have been used to discover the genes underlying this variation in plants. The construction
of functional maps, i.e. composed of genes of known function, is an important component of the candidate gene approach. In
the present paper we report the development of 63 single nucleotide polymorphism markers and 15 single-stranded conformation
polymorphism markers for genes encoding enzymes mainly involved in primary metabolism, and their genetic mapping on a composite
map using two pea recombinant inbred line populations. The complete genetic map covers 1,458 cM and comprises 363 loci, including
a total of 111 gene-anchored markers: 77 gene-anchored markers described in this study, 7 microsatellites located in gene
sequences, 16 flowering time genes, the Tri gene, 5 morphological markers, and 5 other genes. The mean spacing between adjacent markers is 4 cM and 90% of the markers
are closer than 10 cM to their neighbours. We also report the genetic mapping of 21 of these genes in Medicago truncatula and add 41 new links between the pea and M. truncatula maps. We discuss the use of this new composite functional map for future candidate gene approaches in pea. |
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