Regioselectivity enhancement by partial purification of lipase fromAspergillus niger |
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| Authors: | Hao-Ping Chen Kwo-Feng Hsiao Shih-Hsiung Wu Kung-Tsung Wang |
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| Affiliation: | (1) Department of Biochemistry, China Medical College, Taichung, Taiwan;(2) Department of Chemistry, National Taiwan University, Taipei, Taiwan;(3) Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan |
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| Abstract: | Lipase fromAspergillus niger was partially purified by a single step of ion-exchange chromatography and two major fractions I and II could be obtained. Two substrates, peracetylated 1-methyl and 1-thioethyl  -D-glucopyranosides, were deacetylated by fraction II to give 3-OH derivatives as sole products with very high yield, respectively. |
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