The <Emphasis Type="Italic">cnr</Emphasis>-like operon in strain <Emphasis Type="Italic">Comamonas</Emphasis> sp. encoding resistance to cobalt and nickel |
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Authors: | T V Siunova A V Siunov V V Kochetkov A M Boronin |
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Institution: | (1) Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Moscow oblast, Pushchino, 142290, Russia;(2) Pushchino State University, Moscow oblast, Pushchino, 142290, Russia |
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Abstract: | Plasmid pBS501 was detected in the strain Comamonas sp. BS501. This plasmid specifies high level of induced resistance (5 mM) to cobalt/nickel both in the host strain and in related strains C. testosteroni B-1241 and C. acidovorans B-1251. Hybridization analysis revealed a homology of pBS501 restriction fragments with the only well-characterized operon cnrXYHCBAT that resides in plasmid pMOL28 from Cupriavidus metallidurans CH34. Essential differences in the structural organization of the cobalt/nickel resistance determinant were found between plasmid pBS501 and the cnr operon. |
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