Effects of temperature and various metal ions on the solubility of tobacco RuDP carboxylase |
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Authors: | Kawashima, Nobumaro Ayabe, Tomiko |
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Affiliation: | Hatano Tobacco Experiment Station, Japan Monopoly Corporation Hatano, Kanagawa, Japan |
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Abstract: | Tobacco RuDP carboxylase is completely soluble in 0.07 M NaClor 0.01 M Na2SO2, but is almost completely insoluble in salt-freesolutions at 40°C; the solubility seeming to depend on ionicstrength. Lowering the temperature increased solubility of theprotein. The solubility in 0.010.04 M NaCl at 0°Cwas more than double that at 40°C. RuDP solubilized theprotein even in a salt-free medium. The protein became insolubleagain on the addition of various divalent cations. Effectivenessof the metal ions was Zn++> Ni++>Co++>Mn++>Mg++>Ca++.Although most of the metal ions inhibited (Mg++ activated) enzymeactivity, no direct correlation was found between the degreeof solubility depression and the degree of enzyme inhibition. (Received October 4, 1971; ) |
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