Ca2+ transport in mitochondrial and microsomal fractions from higher plants |
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Authors: | Peter Dieter Dieter Marmé |
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Affiliation: | (1) Institut für Biologie III der Universität Freiburg, Schänzlestraße 1, D-7800 Freiburg, Federal Republic of Germany |
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Abstract: | Mitochondria from etiolated corn possess a much greater Ca2+ uptake capacity per mg protein than microsomes from the same source. Differences in energy requirements, sensitivity to specific inhibitors, and sedimentation properties enabled us to study both Ca2+ uptake mechanisms without mutual contamination. The microsomal Ca2+ uptake does not vary much among different plants as compared to the mitochondrial Ca2+ uptake; this is also true for different organs of the same plant. Mitochondrial Ca2+ uptake is more dependent on the age of the seedlings than microsomal uptake, because of changes in active Ca2+ uptake activity rather than of changes in efflux. Intactness and the oxidative and phosphorylative properties of the mitochondria remained unchanged during this time period. Na+ and Mg2+ do not induce Ca2+ release from mitochondria.Abbreviations ATP adenosine triphosphate - ADP adenosine diphosphate - NADH2 -nicotinamide adenin dinucleotide, reduced form - Mops 3-(N-morpholino)propane-sulfonic acid - Tris tris-(hydroxymethyl)-aminomethane - Hepes hydroxyethylpiperazine-N-2-ethanesulfonic acid - BSA bovine serum albumin - EDTA (ethylene-dinitrilo)-tetraacetic acid - EGTA ethylene glycol-bis(-aminoethylether)-N,N-tetraacetic acid - CCCP carbonyl cyanide m-chlorophenylhydrazone - DTE 1,4-dithiothreitol |
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Keywords: | Calcium uptake Microsomes Mitochondria Transport (Ca2+) Zea |
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