| A_（8—10）替换的胰岛素类似物的合成及生物活性 |
| |
| 引用本文： | 杨士珍,林威,黄一丁,冯佑民,钮经义.A_（8—10）替换的胰岛素类似物的合成及生物活性[J].生物化学与生物物理学报,1994(6). |
| |
| 作者姓名： | 杨士珍 林威 黄一丁 冯佑民 钮经义 |
| |
| 作者单位： | 中国科学院上海生物化学研究所 |
| |
| 基金项目： | 中国科学院“八·五”重点科研项目，国家自然科学基金 |
| |
| 摘 要： | 本文报道了用Ｆｍｏｃ固相法合成３种胰岛素Ａ链小环（Ａ８－１０）被不同碱性氨基酸取代的Ａ链类似物，并分别与天然胰岛素Ｂ链重组成相应胰岛素类似物；经受体结合，整体活性及抗体结合实验，均表现出相应的活性。从中可以推测出：Ａ链小环区域不是胰岛素表现生物活性的重要部位，而是胰岛素与其抗体结合较重要的区域。
|
| 关 键 词： | A_（8－10）替换 胰岛素类似物 受体结合 抗原性 |
Synthesis and Activity of Insulin Analogues with Alteration at A_(8-10) |
| |
| YANG Shi-Zhen,LIN Wei,HUANG Yi-Ding,DENG You-Min and NIU Jing-Yi.Synthesis and Activity of Insulin Analogues with Alteration at A_(8-10)[J].Acta Biochimica et Biophysica Sinica,1994(6). |
| |
| Authors: | YANG Shi-Zhen LIN Wei HUANG Yi-Ding DENG You-Min and NIU Jing-Yi |
| |
| Abstract: | Three insulin A-chain analogues with substitution by basic amino acia residues at A8-10 positions inside the intra-disulfide bonds were synthesized by the solid phase methods with Fmoc protection strategy.The corresponding insulin analogues obtained through reconstitution with the B-chain of porcine insulin showed definite activities in receptor binding,mouse convulsion test and antigenicity assays.It is suggested that this region inside A6-11 was not much concerned with biological activities,but rather more important antigenically in the binding to its antibodies. |
| |
| Keywords: | A8-10 substitutions Insulin analogues Receptor binding Antigenicity |
| 本文献已被 CNKI 等数据库收录！ |
