Induction of nonspecific, proliferative dependent suppressor T lymphocytes in human mixed lymphocyte cultures |
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Authors: | J J Rinehart |
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Institution: | 1. Departments of Medical Biology and Parasitology, Jichi Medical School, Tochigi-ken, Japan;2. Department of Clinical Pathology, Jichi Medical School, Tochigi-ken, Japan;3. Department of Otolaryngology, Jichi Medical School, Tochigi-ken, Japan |
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Abstract: | The stimulation of highly purified human T and B cells by soluble and insoluble protein A was studied. Insoluble protein A, such as protein A conjugated to Sepharose beads (S-pro A), or Staphylococcus aureus Cowan I strain bacteria (SpA CoI), markedly stimulated B cells, but did not affect T cells. SpA CoI stimulated B cells independently of the presence of T cells. While soluble protein A failed to stimulate either T or B cells alone, it greatly stimulated the mixture of T and B cells. Mitomycin treatment revealed that the response to soluble protein A was ascribed mainly to the T-cell response with the B-cell helper effect, though partially to the B-cell response with the T-cell helper effect as well. The response of T cells to protein A was enhanced by both the adherent population and the nonadherent B-cell population. This T-B cooperation was mediated by direct cell-to-cell interaction rather than soluble mediators. The binding experiments also demonstrated that the amount of protein A bound to T cells was far less than that to B cells. These results point out the significance of B-cell participation in T-cell activation. The mechanism by which protein A activates T and B cells was also discussed. |
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