DNA cytosine methylation and heat-induced deamination |
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Authors: | Melanie Ehrlich Kyle F. Norris Richard Y. Wang Kenneth C. Kuo Charles W. Gehrke |
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Affiliation: | (1) Department of Biochemistry Tulane Medical School, 70112 New Orleans, LA;(2) Experiment Station Chemical Laboratories, Department of Biochemistry, University of Missouri, 65211 Columbia, MO |
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Abstract: | The heat-induced conversion of 5-methylcytosine (m5C) residues to thymine residues and of cytosine to uracil residues in single-stranded DNA was studied. The calculated rates for deamination at 37°C and pH 7.4 were 9.5×10–10 and 2.1×10–10 sec–1, respectively. N4-Methyldeoxycytidine, which is in the DNA of certain thermophilic bacteria, was more heat-resistant than was deoxycytidine and much more than was 5-methyldeoxycytidine. Thermophilic bacteria which contain N4-methylcytosine rather than m5C in their genomes may thereby largely avoid heat-induced mutation due to deamination, which is incurred by the many organisms that contain m5C in their DNA. |
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Keywords: | DNA 5-methylcytosine mutagenesis |
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