Evidence that Ha-Ras mediates two distinguishable intracellular signals activated by v-Src. |
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Authors: | S A Qureshi K Alexandropoulos M Rim C K Joseph J T Bruder U R Rapp D A Foster |
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Institution: | Institute for Biomolecular Structure and Function, Hunter College, City University of New York, New York 10021. |
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Abstract: | v-Src activates promoters under the control of 12-O-tetradecanoylphorbol-13-acetate (TPA) response elements (TREs) and serum response elements (SREs) via two distinguishable intracellular signaling mechanisms. The induction of TRE- and SRE-mediated gene expression by v-Src could be distinguished by a differential sensitivity to depleting cells of protein kinase C (PKC) and to a dominant negative Raf-1 mutant. Thus, PKC depletion and the dominant negative Raf-1 mutant were able to distinguish two intracellular signaling mechanisms activated by v-Src. Both of these v-Src-induced intracellular signals were sensitive to a dominant negative mutant of Ha-Ras. These data suggest that Ha-Ras functions to coordinately regulate multiple intracellular signaling mechanisms activated by v-Src. |
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