Isolation and characterization of indene bioconversion genes from Rhodococcus strain I24 |
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| Authors: | S L Treadway K S Yanagimachi E Lankenau P A Lessard G Stephanopoulos A J Sinskey |
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| Institution: | (1) Department of Biology, Massachusetts Institute of Technology, Bldg. 68-370, 77 Massachusetts Avenue, Cambridge, MA 02139, USA e-mail: asinskey@mit.edu Tel.: +1-617-253-6721 Fax: +1-617-253-8550, US;(2) Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA, US |
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| Abstract: | Rhodococcus strain I24 is able to convert indene into indandiol via the actions of at least two dioxygenase systems and a putative monooxygenase
system. We have identified a cosmid clone from I24 genomic DNA that is able to confer the ability to convert indene to indandiol
upon Rhodococcus erythropolis SQ1, a strain that normally can not convert or metabolize indene. HPLC analysis reveals that the transformed SQ1 strain produces
cis-(1R,2S)-indandiol, suggesting that the cosmid clone encodes a naphthalene-type dioxygenase. DNA sequence analysis of a portion of
this clone confirmed the presence of genes for the dioxygenase as well as genes encoding a dehydrogenase and putative aldolase.
These genes will be useful for manipulating indene bioconversion in Rhodococcus strain I24.
Received: 8 December 1998 / Received revision: 26 January 1999 / Accepted: 5 February 1999 |
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