Bioactivity of a pentapeptide isolated from corn gluten hydrolysate on Lolium perenne L. |
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Authors: | D. L. Liu N. E. Christians |
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Affiliation: | (1) Department of Horticulture, Iowa State University, 50011 Ames, Iowa, USA |
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Abstract: | Corn gluten hydrolysate (CGH) has been observed to inhibit root formation of germinating grass seeds and has the potential for use as a natural herbicide. Five dipeptides have been isolated from the aqueous solution of CGH and proved to have greater root-inhibiting activity than the crude extract of CGH. The objective of this study was to isolate and identify other biologically active compounds from CGH with herbicidal properties. A perennial ryegrass (Lolium perenne L.) Petri dish bioassay was used to test for the bioactivity. A pentapeptide, Leu-Ser-Pro-Ala-Gln, was isolated from CGH by using Sephadex G-15 gel filtration and two-step C18 reversed phase high performance liquid chromatography procedures. The compound suppressed growth of both the root and the shoot of germinating perennial ryegrass. It required 0.5 mg/mL of the pentapeptide to inhibit 50% of root length in the perennial ryegrass bioassay, and this compound is more active than any of the five dipeptides isolated previously from CGH.Abbreviations CGM corn gluten meal - CGH corn gluten hydrolysate - HPLC high performance liquid chromatography - RP reversed phase - MeOH methanol - AUFS absorbance units of full scale - Rt retention time - TFA trifluoroacetic acid - PTH phenylthiohydantoin - ANOVA analysis of variance |
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Keywords: | Natural products Weed control |
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