In vitro propagation of Fibigia triquetra (DC.) Boiss., a rare stenoendemic species

A rapid clonal propagation method for Fibigia triquetra (DC.) Boiss. (Brassicaceae), a rare Croatian stenoendemic species, has been developed. Shoots originated from aseptically germinated seeds were used for culture initiation. The highest multiplication rate of 9.2 shoots per explant was achieved in a 4-weeks-culture period in the third subculture on half strength Murashige and Skoog medium supplemented with 0.5 µM 6-benzylaminopurine and 2.9 µM gibberellic acid. Excised shoots were rooted best with addition of 8.6 µM indole-3-butyric acid on the same basal medium. Rooted plantlets were successfully transferred to potting soil and acclimatized to outdoor conditions.