Methylmercury modulates GABAA receptor complex differentially in rat cortical and cerebellar membranes in vitro |
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| Authors: | Dr Hannu Komulainen Arto Keränen Veijo Saano |
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| Institution: | (1) Department of Pharmacology and Toxicology, University of Kuopio, P.O.B. 1627, 70211 Kuopio, Finland;(2) Division of Environmental Health, National Public Health Institute, P.O.B. 95, 70701 Kuopio, Finland;(3) Department of Biochemistry and Biotechnology, University of Kuopio, Kuopio, Finland |
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| Abstract: | Effects of methylmercury (MetHg) on the specific 3H]flunitrazepam binding were studied in rat cortical and cerebellar P2-fractions in vitro. MetHg did not affect significantly the specific 3H]flunitrazepam binding in unwashed P2-fraction but increased it marginally (by 16%) at 100  M in washed P2-fraction, in both brain regions.Muscimol (3 M), a GABAA agonist, stimulated the 3H]flunitrazepam binding by 30% to 50% depending on the brain region. In washed cerebellar membranes the enhancing response of muscimol was 10 to 14% lower after preincubation of the tissue with MetHg but in cerebral cortex MetHg did not modulate the muscimol response at all. The results indicate that Met-Hg may have region specific effects on GABAA receptors in vitro and the effect may depend on the occupational state of the GABA binding domain of the receptor complex. |
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| Keywords: | Methylmercury benzodiazepine receptor GABAA receptor flunitrazepam muscimol rat brain |
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