Plant regeneration in pigeonpea [Cajanus cajan (L.) Millsp.] by organogenesis |
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Authors: | M L Mohan K V Krishnamurthy |
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Institution: | (1) Plant Tissue Culture Division, National Chemical Laboratory, Pune – 411 008, India Fax no.: +91-212-349038 E-mail: kvk@ems.ncl.res.in, IN |
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Abstract: | A method for regenerating pigeonpea Cajanus cajan (L.) Millsp.] plants has been developed using distal cotyledonary segments of mature seeds as explants. A large number of
shoot buds were induced directly from explants of genotypes T-15-15 and GAUT-82-90 when cultured on six different basal media
fortified with 22.2 μm N6-benzylaminopurine, 2.3 μm kinetin, and 271 μm adenine sulfate. The shoot buds developed into shoots when they were subcultured on the same medium but with one-tenth concentrations
of cytokinins and adenine sulfate. The shoots elongated by subculturing first two to three times on Murashige and Skoog (MS)
basal medium supplemented with 2.22 μm N6-benzylaminopurine and 0.54 μm
α-naphthaleneacetic acid or on half-strength MS medium containing 2.89 μm gibberellic acid, and then once on the same medium without growth regulators. Elongated shoots were rooted with 80–85% efficiency
on MS medium with 4.92 μm indole-3-butyric acid and the plantlets were transferred for hardening. Plants survival in pots was 70–75%. This method may
be useful for improving the crop through genetic manipulations.
Received: 11 August 1997 / Revision received: 12 January 1998 / Accepted: 30 January 1998 |
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Keywords: | Organogenesis Cotyledon Pigeonpea Plant regeneration |
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