Liquid chromatographic determination of hydroxyproline in tissue samples |
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Authors: | Hutson Paul R Crawford Mark E Sorkness Ronald L |
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Institution: | UW School of Pharmacy, 777 Highland Avenue, Madison, WI 53705-2222 USA. prhutson@pharmacy.wisc.edu |
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Abstract: | We describe a reversed-phase assay of hydroxyproline in rat lung tissue using sarcosine for the internal standard and pre-injection reaction with both o-phthalaldehyde (OPA) and 9-fluorenylmethylchloroformate (FMOC). Intra-assay variability in the concentration range of 25-500 microM hydroxyproline was less than 1%. Normal rat (left) lung was found to have a hydroxyproline content of 1.08+/-0.18 mg/lung. This ability to measure minute amounts of hydroxyproline is being applied to the measure of collagen and pathological fibrosis. |
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