Analysis of kafirin promoter activity in transgenic tobacco seeds |
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Authors: | Richard T. DeRose Dilara Begum Timothy C. Hall |
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Affiliation: | (1) Institute of Developmental and Molecular Biology, Texas A&M University, 77843-3155 College Station, TX, USA;(2) Department of Biology, Texas A&M University, 77843-3155 College Station, TX, USA;(3) Present address: Department of Biotechnology, Rhône-Poulenc Agro, BP 9163, 69263 Lyon Cedex 09, France |
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Abstract: | Sequences corresponding to 855 bp of 5 promoter region and the transit peptide from GK.1, a genomic clone encoding a 22 kDa -kafirin seed protein from sorghum, were translationally fused to a cloned -glucuronidase (GUS) coding sequence from uidA and transferred to tobacco via Agrobacterium tumefaciens-mediated transformation. No GUS expression was detectable at any stage of growth in stems or leaves of these plants. However, GUS expression was detected in both embryo and endosperm tissues of resulting tobacco seeds 10–15 days after flowering. Dissected tissues indicate endosperm expression was localized within the bulk endosperm and not within the parenchyma cell layer underlying the integument. These studies also demonstrate that within dissected tobacco embryos, expression from the kafirin promoter was restricted to the mesocotyl region. |
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Keywords: | /content/v84t95t34qg81163/xxlarge945.gif" alt=" agr" align=" BASELINE" BORDER=" 0" >-prolamin kafirin monocot gene expression transgenic tobacco zein |
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