A note on the production and use of antisera for the detection of part-digested sticklebacks in predator stomach-content samples |
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| Authors: | N Giles R S Phillips |
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| Institution: | School of Sciences, University of Buckingham, Buckingham MK18 1EG;The Wellcome Laboratories for Experimental Parasitology, University of Glasgow, Glasgow G61 1QH |
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| Abstract: | Preliminary experiments indicate that serological techniques provide a useful method of identifying specific prey antigens from the stomach contents of fish predators. Problems with cross-reacting antigens from different prey species can be reduced by absorbing anti-sera with tissue extracts of the cross-reacting species. Very small volumes of part-digested prey tissue are sufficient for identification of the prey species when using agarose diffusion plates (Ouchterlony tests). |
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