Plant regeneration from alginate encapsulated somatic embryos of Asparagus cooperi baker

Somatic embryos of Asparagus cooperi were encapsulated as single embryos approximately 4–6 mm in diameter to produce individual synthetic seeds. The frequency of conversion of artificial seeds to plants was 34%. This frequency was affected by the concentration of calcium chloride, the commercial sources of sodium alginate, and the nutrient medium. The conversion frequency of artificial seeds to seedling plants was 8.3% after storage for 90 days at 2°C.Abbreviations NAA -Naphthaleneacetic acid - ABA Abscisic acid - GA₃ Gibberellic acid - Kn 6-Furfurylaminopurine - MS Murashige and Skoog (1962) medium - SH Schenk and Hildebrandt (1972) medium - WM White (1963) medium