Heat activation/shock temperatures for Bacillus anthracis spores and the issue of spore plate counts versus true numbers of spores |
| |
Authors: | Turnbull Peter C B Frawley Dody A Bull Robert L |
| |
Affiliation: | Biological Defense Research Directorate, Naval Medical Research Center, Silver Spring, MD 20910-7500, USA. peterturnbull@tesco.net |
| |
Abstract: | Assessing true numbers of viable anthrax spores is complex. Optimal heat activation conditions vary with species, media and germinants. Published time/temperature combinations for Bacillus anthracis spores range from 60 degrees C for 1, post-heating counts were less than their pre-heating counterparts on between 71% and 88% of occasions. A high probability was found of viable spore counts differing significantly from counts determined microscopically, with differences of almost 1 log possible. Viable counts were lower than microscopic counts in 15 of 18 tests. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|