Social signals influence hormones independently of calling behavior in the treefrog (Hyla cinerea)

Social signals play an important role in regulating hormone-behavior relationships. In anurans (frogs and toads), acoustic signals are an essential aspect of reproductive behavior; however, the physiological consequences of receiving social signals has remained largely undescribed. Each night for 5, 10, or 20 days, we presented acoustically isolated male treefrogs with a conspecific mating chorus, an array of tones, or no sound. We recorded calling rate of individuals throughout the experiment and collected blood before and after treatment. Days of stimulus exposure had no effect on any dependent measure. Acoustic treatment influenced steroid levels; testosterone, dihydrotestosterone, and corticosterone increased only in the group exposed to the chorus. Chorus-exposed males also showed an increase in stimulus-evoked calling. We found no correlation between androgens and calling within each treatment group. In addition, noncallers in the chorus group had higher levels of androgens than males in the tone or no sound groups. Further, chorus-exposed males with zero, low, or high rate of calling had similar levels of androgens. These data indicate that social signals increase circulating androgens independently of calling behavior. Elevated corticosterone associated with chorus reception did not inhibit calling behavior, and corticosterone showed no correlation with androgen levels.