球孢白僵菌线粒体序列分析及系统进化树构建

线粒体是真核细胞的重要的细胞器,在细胞能量代谢和细胞衰老方面扮演了重要角色,同时也是研究物种进化关系的重要资源。本文应用球孢白僵菌的线粒体序列分析了白僵菌与粪壳菌纲其他11属的13种真菌间的进化关系,为阐明球孢白僵菌的进化地位提供了新的论据。线粒体编码的rnl,rns,25个tRNAs和14个蛋白质基因均由同一条链编码,大部分的tRNA分布为三个tRNA簇,这与粪壳菌纲的其他物种相似。与NCBI上公布的序列比对发现球孢白僵菌线粒体序列与蝇蚧霉线粒体序列最相似,相似率达73%,用14个蛋白编码基因通过邻接法(Neighbor-joint)和最大简约法(Maximum parsimony)构建的系统进化树也得到相同结论,其支持率均为100%。

Sequence and phylogenetic analysis of Beauveria bassiana with mitochondrial genome

Mitochondria are important cytoplasmic organelles of eukaryotic cells in energy metabolism and cell aging. Gene sequences of mitochondrial DNA (mtDNA) are also an important candidate for phylogenetical study. In the present paper, the whole mitochondrial DNA sequence of Beauveria bassiana, registered in EMBL with the accession number EU371503, was used to clarify the relationship between B. bassiana and 13 fungal species in 11 genera including Lecanicillium, Metarhizium, Hypocrea, Fusarium, Gibberella, Verticillium, Neurospora, Podospora, Penicillium, Aspergillus, and Trichophyton of Pezizomycotina. The result showed that rnl, rns, 25 tRNAs and 14 protein genes of B. bassiana were all encoded by the sequences on the same strand and the most of the tRNAs arranged in three conserved trn gene clusters, revealing similarities to other members of Sordariomycetes. Phylogenetic analysis demonstrated that B. bassiana was highly similar to Lecanicillium muscarium based on the whole mtDNA sequence with querage coverage at 73%, and on 14 protein-coding genes with 100% bootstrap support by both NJ method and MP method.