Human colorectal tumours in short-term organ culture A stathmokinetic study |
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| Authors: | C. J. Pritchett,P. V. Senior,J. P. Sunter,A. J. Watson,D. R. Appleton&dagger ,R. G. Wilson |
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| Affiliation: | *Departments of Surgery Pathology, University of Newcastle upon Tyne;†Medical Statistics, University of Newcastle upon Tyne |
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| Abstract: | Abstract. Short-term organ culture, using a technique to preserve epithelial/stromal interaction and metabolism, is a useful technique for carrying out kinetic studies on human colorectal carcinoma and adjacent normal mucosa, providing initial perturbations of proliferative indices are allowed to settle. Tumours require 3.0 μg/ml vincristine for complete metaphase arrest compared with mucosa, which needs 0.5 μg/ml, a 6-fold difference. Using a stathmokinetic technique, the birth rate of tumour cells is 10.21 cells/1000 cells per hr, compared with 7.73 cells/1000 cells per hr for mucosa, a statistically significant difference ( P < 0.01). |
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