细薄星芒海绵中活性菌筛选及混合菌协同效应

通过平板涂布法从我国南海三亚海域细薄星芒海绵中筛选出104株海洋细菌，采用琼脂扩散法、纸片法和细胞浓度记数法进行抗菌活性筛选，发现23株对于大肠埃希氏菌、金黄色葡萄球菌、荧光假单胞菌、枯草芽孢杆菌、黑曲霉、白假丝酵母、宛氏拟青霉具有稳定的抗菌活性，占总细菌的22．2％；根据形态学与生化指标分析初步确认其中4株抗菌活性显著的A05、A08、A72、A75为芽孢杆菌。研究发现，海绵细菌在抗菌活性方面具有正向和负向的协同效应，其中A72-75组合对于白假丝酵母和荧光假单胞菌具有显著的正向协同效应。

Screening of Sponge Stelletta tennui Associated Anti-microbial Bacteria and Their Synergistic Effects

A total of 104 marine bacterial strains were isolated from Stelletta tennui around Sanya area of South China Sea by dilution-plate method and were screened for anti-microbial activity on Escherichia coli, Staphylococcus attreu, Pseudomonas fluorescens , Bacillus subtilis, AspergiUus niger, Candida albicans and Pacecilomyces variotii by agar diffusion, paper disc diffusion assay and cell concentration counts methods. It was found that 23 strains, which are 22. 2% of the total isolated strains, have anti-microbial activities. Among the 23 strains, A05, A08, A72 and A75 were morphologically, physiologically and biochemically characterized and identified to be the genus Bacillus. At the same time, it was proved that there are positive and negative synergistic effects between or among active strains, e. g. , as for A72-75 combination, an obvious enhanced anti-microbial activities on inhibiting Candida albicans and Pseudomomas fluorescens growth was observed than A72 or A75.