Determination of the quaternary structure of protocatechuate 3,4-dioxygenase from Pseudomonas aeruginosa |
| |
Authors: | D H Ohlendorf P C Weber J D Lipscomb |
| |
Institution: | Central Research and Development Department, E. I. du Pont de Nemours & Company, Wilmington, DE 19898. |
| |
Abstract: | A 2.5 A resolution data set has been collected for crystals of protocatechuate 3,4-dioxygenase from Pseudomonas aeruginosa. Analysis of the data using the rotation function shows that the alpha 2 beta 2 tetramers associate to form a particle with cubic 23 (T) point group symmetry. Prior to this analysis it was believed that eight tetramers associated to form the holoenzyme. The symmetry of the crystalline holoenzyme also addresses questions concerning its iron content and substrate stoichiometry. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |