Secretion and hydrolysis of ATP by Ehrlich ascites tumor cells during centrifugation. Dependence on calcium and temperature |
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Authors: | A M Krasnyi A I Sergeev L P Dolgacheva E L Nikiforov V P Zinchenko |
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Institution: | 1.Institute of Cell Biophysics,Russian Academy of Science,Pushchino, Moscow oblast,Russia;2.Pushchino State University,Pushchino, Moscow oblast,Russia |
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Abstract: | Precipitation of Ehrlich ascites tumor cells (EATC) by centrifugation causes ATP secretion. ATP secretion is accompanied by
an increase of calcium concentration in the cytosol and persists for a long time (minutes) after centrifugation during the
storage of cells at a low temperature. During prolonged storage (for more than 1.5 h), the concentration of extracellular
ATP decreases to the level of ∼100 nM due to termination of secretion and ATP hydrolysis by surface ATPases. The rate of ATP
hydrolysis exponentially falls with the temperature decrease from 36 to 8°C. ARL67156, a selective inhibitor of E-NTPDase-1,
effectively suppresses the extracellular ATP hydrolysis. The intensity of ATP secretion does not correlate with the calcium
ions concentration in the cytosol and Ca2+ mobilization from endoplasmic reticulum but correlates with the intensity of Ca2+ influx into the cells. The temperature dependences of ATP secretion intensity and Ca2+ entry rate coincide. |
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