| 黄霉素高产菌株的选育 |
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| 引用本文： | 王洪秀,高树仁,童应凯,杨永涛,韦东胜.黄霉素高产菌株的选育[J].生物技术,2006,16(6):26-29. |
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| 作者姓名： | 王洪秀 高树仁 童应凯 杨永涛 韦东胜 |
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| 作者单位： | 1. 黑龙江八一农垦大学,黑龙江,大庆,163319
2. 天津农学院,天津,300384 |
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| 摘 要： | 目的:为了获得黄霉素高产菌株。方法:进行了一系列的诱变筛选。以加纳链霉菌(Streptomyces ghanaesis)SgWE-11为出发菌株,采用紫外线诱变,以其自身代谢物黄霉素作为筛选因子。结果:获得一株遗传稳定且黄霉素发酵效价比出发菌株高出170.25%的菌株。对该菌株采用亚硝酸诱变,并以链霉素作为筛选因子时得到一株效价值为875u/mL的高产菌株SgWE-25,比出发菌株SgWE-11提高了4.4倍。传代结果表明,在传代的同时结合自然分离,可以保持稳定的产抗特性。
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| 关 键 词： | 黄霉素 紫外线诱变 亚硝酸诱变 |
| 文章编号： | 1004-310X（2206）06-0026-04 |
| 收稿时间： | 2006-06-09 |
| 修稿时间： | 2006-09-07 |
Improvement of Flavomycin Production in Streptomyces ghanaesis WE- 11 by Combination Mutant |
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| WANG Hong-xiu,GAO Shu-ren,TONG Ying-kai,YANG Yong-tao,WEI Dong-sheng.Improvement of Flavomycin Production in Streptomyces ghanaesis WE- 11 by Combination Mutant[J].Biotechnology,2006,16(6):26-29. |
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| Authors: | WANG Hong-xiu GAO Shu-ren TONG Ying-kai YANG Yong-tao WEI Dong-sheng |
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| Institution: | 1. HLJ August First Reclamation University, Daqing 163319, China; 2. Tianjin Agricultural University , Tianjin 300384, China |
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| Abstract: | Objective:In order to get some high-yield strains,different mutant modes and screening factors were used.Method:A flavomycin producing strain SgWE-11 was treated by UV and flavomycin as screening factor.Results:A stable strain was obtained with the titer of flavomycin increased by 170.25% than parent strain.Then the mutant which grow on media containing Streptomycin was treated with HNO_2,we got a high-yeild strain SgWE-25.The productivity of strain SgWE-25 reached 875u/mL which was 4.4-times.higher than that of the original of strain SgWE-11.When it was subcultured combined with natural screening,its stability was rather satisfied. |
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| Keywords: | Flavomycin UV mutagenesis HNO_2 mutagenesis |
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