Dynamic expression of immune response genes in the sea bass, Dicentrarchus labrax, experimentally infected with the monogenean Diplectanum aequans |
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Authors: | Faliex Elisabeth Da Silva Catherine Simon Gaël Sasal Pierre |
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Affiliation: | Laboratoire de Biologie et d'Ecologie Tropicale et Méditerranéenne, UMR 5244, CNRS, EPHE, UPVD, Université de Perpignan, 58 avenue Paul Alduy, 66860 Perpignan Cedex, France. |
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Abstract: | The sea bass, Dicentrarchus labrax, is one of the most extensively farmed marine fishes in the Mediterranean. Under the high-density condition common in aquaculture, the monogenean gill parasite Diplectanum aequans can cause significant economic losses. This study used real-time quantitative PCR to investigate the dynamic expression of immune response genes in sea bass infected with Diplectanum aequans. The target genes, interleukin-1 (IL-1beta, transforming growth factor (TGF-beta and T-cell receptor (TCR-beta), were studied in the gills and spleen of the sea bass from the first day of infection until thirty days post- infection. Our results showed that there was an increase in IL-1beta gene expression in the spleen and gills and in TGF-beta gene expression in the gills of infected fish. These results show that parasitic infection induced a local inflammatory reaction and that reaction was restricted to the site of infection. Finally, the absence of relationship between TCR-beta expression and the parasitic infection suggests that the adaptive immune system is not involved in the response against this parasite. |
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Keywords: | Sea bass Parasite Diplectanum aequans Aquaculture Immunity Real-time PCR Gene expression |
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