Date Palm DNA Mini-Preparation without Liquid Nitrogen |
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| Authors: | Ouenzar Bouchra Hartmann Caroline Rode Andre Benslimane Abdelali |
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| Affiliation: | (1) Département de Biologie, Faculté des Sciences Semlalia, Université Cadi Ayyad, BP S15, 40001 Marrakech, Morocco;(2) Institut de Biotechnologie des Plantes, ERS CNRS 569, Bâtiment 630, Université de Paris Sud, F-91405 Orsay, France |
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| Abstract: | We have developed a new mini-procedure for isolation of total cellular DNA from date palm (Phoenix dactylifera L.). The procedure, which does not use liquid nitrogen, has proved useful due to temporary disruptions in supplies of liquid nitrogen that occur in countries where date palm trees are cultivated. DNA suitable for RFLP and PCR analyses is obtained. |
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| Keywords: | date palm DNA isolation liquid nitrogen |
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