"In vivo" (35S)methionine interaction with rat liver tRNA |
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| Authors: | D Kanduc M R Rossiello T Ranieri E Quagliariello |
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| Affiliation: | Dipartimento di Biochimica e Biologia Moleculare, Facoltá di Scienze, Universitá di Bari, Italy. |
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| Abstract: | As part of a study to characterize the methionine role in tumorigenesis, we report that methionine sulfur interacts with rat liver tRNA "in vivo" (35S) radioactivity remained associated to the nucleic acid after a number of treatments, including tRNA deacylation. Similar data were obtained after administration of (methyl-3H) methionine, while no comparable tRNA labelling was detected when the aminoacid labelled in the aliphatic chain was given. Hplc analysis of (35S) tRNA enzymic hydrolysate showed two unidentified UV-absorbing radioactive peaks. NMR spectra of these two peaks did not reveal any thiomethyl group. |
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