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A genetic linkage map of Nicotiana plumbaginifolia/ Nicotiana longiflora based on RFLP and RAPD markers |
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| Authors: | T Y Lin Y Y Kao S Lin R F Lin C M Chen C H Huang C K Wang Y Z Lin C C Chen |
| Institution: | Department of Life Science, National Tsing Hua University, Hsinchu, Taiwan, Republic of China, TW Department of Botany, National Taiwan University, Taipei, Taiwan, Republic of China e-mail: chencc@ccms.ntu.edu.tw Tel.: +886-2-23630231 ext. 2371, Fax: +886-2-23918940, TW Institute of Botany, Academia Sinica, Taipei, Taiwan, Republic of China, TW |
| Abstract: | We have constructed a genetic linkage map for Nicotiana plumbaginifolia/Nicotiana longiflora (2n= 2x=20), based on the segregation of 69 RFLP and 102 RAPD loci in 99 F2 plants from the cross N. plumbaginifolia×N. longiflora. The map consists of nine major linkage groups, each containing more than nine marker loci, and spans 1062 cM. Twenty of the RFLP markers were mapped previously to Nicotiana sylvestris (2n=2x=24) chromosomes using monosomic alien addition lines. Taxonomically, N. plumbaginifolia and N. sylvestris belong to the same section, namely the Alatae; however, cytogenetic evidence indicates that they are not closely related. Comparison of the distribution of markers common to both maps suggests that genome reorganization has occurred during the evolution of these two species. Evidence is also presented that genome reorganization may be accompanied by gain and loss of specific classes of DNA sequences in their genomes. |
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