Biodiversity in deep-sea sites located near the south part of Japan |
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Authors: | H Takami Kuniko Kobata Takahiko Nagahama Hideki Kobayashi Akira Inoue Koki Horikoshi |
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Institution: | (1) Deep-Sea Microorganisms Research Group, Japan Marine Science and Technology Center, 2-15 Natsushima-cho, Yokosuka 237-0061, Japan Tel. +81-468-67-3895; Fax +81-468-66-6364 e-mail: takamih@jamstec.go.jp, JP |
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Abstract: | We obtained 100 isolates of bacteria from deep-sea mud samples collected at various depths (1050–10 897 m). Various types
of bacteria such as alkaliphiles, thermophiles, psychrophiles, and halophiles were recovered on agar plates at a frequency
of 0.8 × 102 to 2.3 × 104/g of dry sea mud. No acidophiles were recovered. These extremophilic bacteria were widely distributed, being detected at
each deep-sea site, and the frequency of isolation of such extremophiles from the deep-sea mud was not directly influenced
by the depth of the sampling sites. Phylogenetic analysis of deep-sea isolates based on 16S rDNA sequences revealed that a
wide range of taxa were represented in the deep-sea environments. Growth patterns under high hydrostatic pressure were determined
for the deep-sea isolates obtained in this study. No extremophilic strains isolated in this study showed growth at 60 MPa,
although a few of the other isolates grew slightly at this hydrostatic pressure.
Received: August 3, 1998 / Accepted: October 20, 1998 |
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Keywords: | Extremophiles Halophiles Psychrophiles Alkaliphiles Thermophiles Deep sea 16S ribosomal RNA Phylogenetic tree |
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