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Analysis of the role of Ser1/Ser2/Thr9 phosphorylation on myosin II assembly and function in live cells
Authors:Jordan R Beach  Lucila S Licate  James F Crish  Thomas T Egelhoff
Affiliation:1. Department of Hematology, the First Affiliated Hospital of Jinan University, Guangzhou, 510630, China
2. Department of Hematology, Guangdong No.2 Provincial People’s Hospital, Guangzhou, 510317, China
3. Department of Internal Medicine, the Third Affiliated Hospital of Nanfang Medicine University, Guangzhou, 510630, China
4. Institute of Life and Health Engineering, Jinan University, Guangzhou, 510630, China
5. Department of Plastic Surgery and Cosmetology, the First Affiliated Hospital, Jinan University, Guangzhou, 510630, China
Abstract:

Background

The therapeutic efficacy of human mesenchymal stem cells (hMSCs) for the treatment of hypoxic-ischemic diseases is closely related to level of hypoxia in the damaged tissues. To elucidate the potential therapeutic applications and limitations of hMSCs derived from human umbilical cords, the effects of hypoxia on the morphology and proliferation of hMSCs were analyzed.

Results

After treatment with DFO and CoCl2, hMSCs were elongated, and adjacent cells were no longer in close contact. In addition, vacuole-like structures were observed within the cytoplasm; the rough endoplasmic reticulum expanded, and expanded ridges were observed in mitochondria. In addition, DFO and CoCl2 treatments for 48 h significantly inhibited hMSCs proliferation in a concentration-dependent manner (P < 0.05). This treatment also increased the number of cells in G0/G1 phase and decreased those in G2/S/M phase.

Conclusions

The hypoxia-mimetic agents, DFO and CoCl2, alter umbilical cord-derived hMSCs morphology and inhibit their proliferation through influencing the cell cycle.
Keywords:
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