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Pseudonocardia hispaniensis sp. nov., a novel actinomycete isolated from industrial wastewater activated sludge |
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| Authors: | G. Cuesta A. Soler J. L. Alonso M. A. Ruvira T. Lucena D. R. Arahal M. Goodfellow |
| Affiliation: | 1. área de Microbiología, Departamento de Biotecnología, E.T.S.I. Agronómica y Medio Natural, Universitat Politècnica de València, Camino de Vera 14, 46022, Valencia, Spain 2. Instituto Universitario de Ingeniería del Agua y Medio Ambiente, Universitat Politècnica de València, 46022, Valencia, Spain 3. Colección Espa?ola de Cultivos Tipo (CECT) and Departamento de Microbiología y Ecología, Universidad de Valencia, Campus de Burjassot-Paterna, Valencia, Spain 4. School of Biology, Newcastle University, Newcastle upon Tyne, NE1 7RU, UK |
| Abstract: | A novel actinomycete, designated PA3T, was isolated from an oil refinery wastewater treatment plant, located in Palos de la Frontera, Huelva, Spain, and characterized taxonomically by using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate formed a distinct subclade in the Pseudonocardia tree together with Pseudonocardia asaccharolytica DSM 44247T. The chemotaxonomic properties of the isolate, for example, the presence of MK-8 (H4) as the predominant menaquinone and iso-C16:0 as the major fatty acid, are consistent with its classification in the genus Pseudonocardia. DNA:DNA pairing experiments between the isolate and the type strain of P. asaccharolytica DSM 44247T showed that they belonged to separate genomic species. The two strains were readily distinguished using a combination of phenotypic properties. Consequently, it is proposed that isolate PA3T represents a novel species for which the name Pseudonocardia hispaniensis sp. nov. is proposed. The type strain is PA3T (= CCM 8391T = CECT 8030T). |
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