Distinction between vascular smooth muscle cells and myoepithelial cells in primary monolayer cultures of human breast tissue |
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Authors: | O W Petersen B Van Deurs |
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Institution: | (1) Structural Cell Biology Unit, Department of Anatomy, The Panum Institute, DK-2200 Copenhagen N, Denmark;(2) Laboratory of Tumor Endocrinology, The Fibiger Institute, DK-2100 Copenhagen O, Denmark |
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Abstract: | Summary We report on the discrimination of vascular smooth muscle cells and myoepithelial cells in primary cultures of human breast
tissue. Breast tissue was disaggregated enzymatically and the resulting organoids seeded in monolayer culture on collagen-coated
plastic in serum-free medium CDM3a. Two main types of organoids were present after enzymatic digestion. One resembled small
blood vessels and the other interlobular ducts or acini of the breast gland epithelium. Within 3 to 8 d after plating the
organoids migrated into typical monolayer islets. These monolayer islets were evaluated using phase contrast microscopy and
further tagged with monoclonal antibodies for immunocytochemical demonstration of Factor VIII-related antigen, muscle iso-forms
of actin, type IV collagen, vimentin, desmin, and keratins. It is concluded that vascular smooth muscle cells resembled myoepithelial
cells by expressing vimentin filaments, depositing type IV collagen, and showing immunoreactivity to muscle iso-forms of actin.
However, whereas vascular smooth muscle cells were associated with endothelial cells and sometimes expressed desmin, myoepithelial
cells appeared together with luminal epithelial cells and expressed cytokeratins.
This work was supported by the Danish Medical Research Council, the Danish Cancer Society, the NOVO Foundation, and the Thaysen
Foundation. |
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Keywords: | vascular smooth muscle cells myoepithelial cells human breast gland primary cultures |
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