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Effect of dibutyryl cyclic AMP on the restoration of contact inhibition in tumor cells and its relationship to cell density and the cell cycle
Authors:R W Teel  R G Hall
Institution:Department of Physiology, School of Medicine, Loma Linda University, Loma Linda, Calif. 92354, USA
Abstract:KB cell cultures exposed to 10?4 M dibutyryl cyclic AMP were significantly inhibited and exhibited contact inhibition of growth at cell densities of 8 × 104/cm2 irrespective of the initial plating density. Control cultures reached densities of 2.5 × 105/cm2. Inhibition of growth did not occur in KB cells when the density was below 1 × 104 cells/cm2. When dibutyryl cyclic AMP was removed from KB cells in the contact-inhibited state, growth resumed with DNA synthesis beginning in about 6 h. Labeled metaphases increased rapidly after 22 h without the appearance of an early rise in unlabeled metaphases. This suggests that the inhibitory effect of dibutyryl cyclic AMP is on the G1 phase of the cell cycle.
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