An enzyme-bound linamarin indicator paper strip for the semi-quantitative estimation of linamarin

Summary An enzyme-bound linamarin indicator paper strip was developed which was based on the hydrolysis of linamarin by cassava leaf linamarase and the detection of the cyanide released by alkaline picrate reagent. The linamarase could be stabilized with gelatin or gelatin in combination with polyvinylpyrrolidone-10 or trehalose. A positive reaction was observed within 15 minutes at 37°C and it could detect linamarin concentration as low as 0.5 to 1 mM. The indicator strip could be used to estimate linamarin content in cassava semiquantitatively.