Studies on carbohydrate moieties of the glycoprotein,glucoamylase II ofAspergillus niger: Nature of carbohydrate-peptide linkage and structure of oligosaccharides |
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Authors: | P Manjunath M R Raghavendra Rao |
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Institution: | (1) Discipline of Biochemistry and Applied Nutrition, Central Food Technological Research Institute, 570 013 Mysore;(2) Present address: Reproduction Research Laboratory, Clinical Research Institute of Montreal, 110, Pine Avenue, H2W 1R7 West, Montreal, Que., Canada |
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Abstract: | Electrophoretically homogeneous type 1 (GP-C1 and GP-C2), type 2 (GP-C3a and GP-C3b,) and type 3 (GP-D1, and GP-D2) glycopeptides fromAspergillus niger glucoamylase II (Manjunath and Raghavendra Rao, preceding paper) were separately treated with alkaline borohydride. The (\-eliminated
oligosaccharides were subjected to single and sequential digestion with specific glycosidases and the products analysed by
gas liquid chromatography. The studies revealed that carbohydrate moieties were present as mannose, Man-Man-, and trisaccharide
structures, namely, (a) GIc-Man-Man-, (b) Gal-Man-Man, (c) Man-Man-Man-, (d) GlcNAc-Man-Man-, and (e) Xyl-Man-Man. None of
the glycopeptides contained all the trisaccharide structures (a) to (e). Type 1 glycopeptide contained structures (a), (b)
and (c); type 2, (a) and (d) and type 3, (a), (b) and (e). The number of carbohydrate units (mono-, di-and trisaccharides)
present in the major glycopeptides was determined and tentative structures for the glycopeptides proposed. Carbohydrate units
appeared to occur in clusters of 4 to 7 in each glycopeptide, a structure unique to the carbohydrate moiety inAspergillus niger glucoamylase. Based on carbohydrate analysis and yields of glycopeptide, the number of units of each type of glycopeptide
present in glucoamylase II was tentatively calculated to give two of type Man:Glc:Gal = 12–15:l:l, one of type Man:Glc:GlcN
= 10-l1:1:2 and one of type Man :GIc :Gal:Xyl = 4–8:0.1:0.5-0.8:0.3-1 glycopeptides. |
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Keywords: | Glucoamylase Aspergillus niger glycopeptide carbohydrate-peptide linkage central heterogeneity |
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