Direct measurement of the fast rate of double helix formation |
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| Authors: | T M Hickey E Hamori |
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| Affiliation: | 1. Structural and Computational Biology Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany;2. Electron Microscopy Core Facility, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany;3. Leica Microsystems (Schweiz) AG, Max Schmidheiny-Strasse 201, 9435 Heerbrugg, Switzerland;4. Leica Microsystems GmbH, Am Friedensplatz 3, 68165 Mannheim, Germany;5. Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany;6. Molecular Medicine Partnership Unit, EMBL/Universitätsklinikum Heidelberg, Heidelberg, Germany;7. Leica Mikrosysteme GmbH, Hernalser Hauptstraße 219, 1170 Vienna, Austria;1. Department of Microbial Sciences, Institute of Biology, Leiden University, Sylviusweg 72, 2333 BE Leiden, The Netherlands;2. Centre for Microbial Cell Biology, Leiden University, Sylviusweg 72, 2333 BE Leiden, The Netherlands |
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| Abstract: | In a stopped-flow apparatus, a random-coil-to-double-helix conformation change was initiated in aqueous poly d(AT) solutions by a rapid pH change. The rate of formation of the double-stranded helical structure was followed spectrophotometrically at 260 mμ. It was found that two simple first-order processes accounted for the entire reaction. The measured rate constants at 10 °C were 280 and 0.26 sec−1, respectively. |
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