Structural and thermodynamic behavior of cytochrome c assembled with glutathione-covered gold nanoparticles |
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Authors: | Eva Valušová Peter Švec Marián Antalík |
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Institution: | (1) Department of Biophysics, Institute of Experimental Physics, Slovak Academy of Sciences, Watsonova 47, 040 01 Košice, Slovakia;(2) Institute of Physics, Slovak Academy of Sciences, Dúbravská cesta 9, 845 11 Bratislava, Slovakia;(3) Department of Biochemistry, Faculty of Science, P.J. Šafárik University, Moyzesova 11, 040 01 Košice, Slovakia |
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Abstract: | The conformational changes of horse heart ferricytochrome c (cyt c) after association of gold nanoparticles have been studied by electronic absorption spectroscopy and circular dichroism (CD).
Our results show that the structural stability around the heme of complexed cyt c was increased successfully. Glutathione-layered gold nanoparticles caused a significant increase of the apparent pK values of the cyt c alkaline transition. Similarly, the heme crevice became more stable to heat after assembly of cyt c with gold nanoparticles. In contrast, gold nanoparticles weaken the overall thermal stability of the cyt c by decreasing the denaturation temperature estimated from far-UV CD measurements. Similar behavior has previously been reported
for cyt c complexed with physiological redox partners as well as hydrophilic polyanions.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
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Keywords: | Gold nanoparticles Cytochrome c Conformational stability |
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