籼稻明恢63成熟种子愈伤组织的诱导及转基因水稻的抗性检测

利用不含附加营养成分的2,4-D培养基（2mg/L）诱导明恢63的成熟种子，9天预诱导后获得了大量的愈伤组织。利用基因枪辅助的土壤农杆菌转化法将天花粉蛋白（Trichosanthin, TCS）基因转入籼稻明恢63的愈伤组织，并通过再生（含有3mg/L 6-BA, 0.5mg/L ABA和1mg/L NAA的N6培养基）获得了转基因植物。 Southern blot分析和Western blot检测证明外源基因已经插入到T0代明恢63的基因组中并获得了表达。初步研究结果表明，转基因水稻对稻瘟病菌侵染具有抗性。

Callus Induction and Regeneration from Mature Seeds of Indica Rice Minghui 63 and Anti-funga] Assay of Transgenic Rice Plants

A large number of callus from mature seeds of indica rice minghui 63 were obtained through pre-induction on medium with 2mg/L 2,4-D but without inorganic and organic components for 9 days. Trichosanthin gene was transferred into indica rice minghui 63 by using agrobacterium with the help of bombardment and the transgenic plants were obtained by inducing regeneration. Southern and Western blot analysis showed that the trichosanthin gene had been transferred into genome of minghui 63 and expressed in rice plants. The anti-fungal assay suggested that transgenic rice plants enhanced resistance to infection of Pyricularia oryzae.