Determination of diazolidinyl urea in a topical cream by high-performance liquid chromatography |
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Authors: | Robert O. Williams III Vorapann Mahaguna Mongkol Sriwongjanja |
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Affiliation: | Pharmaceutics Division, College of Pharmacy, University of Texas, Austin, TX 78712-1074, USA |
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Abstract: | A high-performance liquid chromatographic method for the determination of diazolidinyl urea (DU) in a cream formulation is described. The aqueous phase of the emulsion was separated by centrifugation, removed, filtered, diluted and applied onto the HPLC system. DU was detected by ultraviolet absorption at a wavelength of 214 nm. The calibration curve was linear over the range of 79–553 μg/ml, and identical when determined on consecutive days. The relative standard deviation for repeat determinations was less than 0.5%. Recoveries were 97.74–101.72%. This analytical method is useful for quantitation of DU in cream formulations. |
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Keywords: | Diazolidinyl urea |
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