| 巴西橡胶树顺式异戊烯基转移酶基因cDNA克隆及其序列特征分析 |
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| 引用本文： | 罗明武,邓柳红,易小平,曾会才,肖苏生. 巴西橡胶树顺式异戊烯基转移酶基因cDNA克隆及其序列特征分析[J]. 热带亚热带植物学报, 2009, 17(3): 223-228 |
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| 作者姓名： | 罗明武 邓柳红 易小平 曾会才 肖苏生 |
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| 作者单位： | 海南大学材料与化工学院,海南,儋州,571737;中国热带农业科学院热带生物技术研究所,农业部热带作物生物技术重点开放实验室,海口,571101 |
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| 基金项目： | 中国热带农业科学院中央级公益性科研院所专项基金,华南热带农业大学科技基金 |
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| 摘 要： | 以巴西橡胶树(Hevea brasiliensis)胶乳的RNA为Tester;叶片RNA为Driver,利用抑制消减杂交法(suppressive subtractive hybridization,SSH)构建了一个胶乳特异表达基因差减文库.通过反式Northern点杂交(reverse Northern dot blots)筛选到一个与顺式异戊烯基转移酶基因(橡胶生物合成的关键酶基因)高度同源的阳性克隆R363.采用RACE方法获得该克隆的全长cDNA(GenBank登陆号:AY461414).序列分析表明,该基因长1156 bp,含有873 bp的阅读框,编码290个氨基酸,分子量约为32.9 kD,等电点为7.2,含有N-端跨膜螺旋区.同源性分析表明R363编码的蛋白质具有异戊烯基转移酶家族的特征,含有cis-异戊烯基链转移酶的5个高度保守区,推测R363可能是一种新的顺式-异戊烯基转移酶基因.Northern blot分析显示,R363在胶乳中高度表达,在叶中不表达.乙烯处理前后表达强度一致,表明该基因表达不为乙烯所诱导.
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| 关 键 词： | 巴西橡胶树 抑制消减杂交法 胶乳 cis-异戊烯基转移酶 |
| 收稿时间： | 2008-07-25 |
| 修稿时间： | 2009-02-20 |
Cloning and Sequence Analysis of a Novel Cis-prenyltransferases Gene from Hevea brasiliensis |
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| LUO Ming-wu,DENG Liu-hong,YI Xiao-ping,ZENG Hui-cai and XIAO Su-sheng. Cloning and Sequence Analysis of a Novel Cis-prenyltransferases Gene from Hevea brasiliensis[J]. Journal of Tropical and Subtropical Botany, 2009, 17(3): 223-228 |
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| Authors: | LUO Ming-wu DENG Liu-hong YI Xiao-ping ZENG Hui-cai XIAO Su-sheng |
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| Affiliation: | 1.College of Materials Science and Chemical Engineering;Hainan University;Danzhou;571737;China;2.Key Laboratory of Tropical Crop Biotechnology;Ministry of Agriculture;Institute of Tropical Bioscience and Biotechnology;Chinese Academy of Tropical Agricultural Sciences;Haikou 571101;China |
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| Abstract: | Cis-prenyltransferases is the key enzyme to rubber biosynthesis.A cDNA fragment which was highly homologous to the gene encoding cis-prenyltransferases was isolated by screening of a latex subtracted cDNA library.The latex subtracted cDNA library was constructed by suppressive subtractive hybridization(SSH),in which the polyA+RNA of latex from Hevea brasiliensis served as tester,and polyA+RNA of leaves as driver.According to its sequences information,a novel full-length cDNA of 1 156 bp termed R363(GenBank ... |
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